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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with either TGF1 10ng/ml (Peprotech, Dollard des Ormeaux, Quebec, Canada), GHK 10 nM (Sigma, Markham, Ontario, Canada) or control media (1% FBS DMEM, penicillin, streptavidin) for a further 48 h
Gross Clinical Assessment Assessment included edema (involving the face, upper and lower lip, snout, paws and scrotum (presented with extreme cyanosis)) that may be prominent, scored from 0 to 3 (0without swelling, 1mild swelling, 2moderate swelling and 3severe swelling), poor respiration as respiratory rate [5] and the number of fatalities after one or two applications of isoprenaline
Aortic stiffness: epidemiology, risk factors, and relevant biomarkers
L-
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